elisa test kits Search Results


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KCAS Bioanalytical and Biomarker Services kcas bio analytical
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TechLab Inc elisa test kits
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R-Biopharm kits elisa
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Cosmo Bio USA rodent fsh elisa test kit
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Biopharm GmbH ridascreen elisa test kits (streptomycin art: r3103)
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KEHUA TECH elisa test kits (kehua)
Nuclear hormone receptors activate HBV replication in 293T cells. (A) HNF4α, RXRα, and PPARα expression plasmids were transfected into 293T cells. After 72 h, mRNA expression levels were quantified by qPCR. Control cells were untransfected 293T. ATRA at 1 μmol/L and clofibric acid at 1 mmol/L, were used as ligands to activate the RXRα and PPARα nuclear hormone receptors, respectively. Plasmid pBR322‐HBV1.0 (0.5 μg) was transfected into cells together with or without nuclear hormone receptors (HNF4α 0.2 μg, RXRα 0.2 μg, PPARα 0.2 μg). (B) At 72 h after transfection, HBV cccDNA was quantified by absolute quantitative PCR, and then calculated as copies per cell. (C) HBV pgRNA was detected by RT‐qPCR. <t>(D)</t> <t>HBsAg</t> was detected by time‐resolved fluorescence. (E) HBeAg was detected by <t>ELISA.</t> (F) HBV DNA in the supernatant was extracted and quantified using an HBV DNA real‐time PCR Assay kit. Results are the means ± SD of three repeats, with differences assessed using Student's t test (* P < .05, ** P < .01, *** P < .001)
Elisa Test Kits (Kehua), supplied by KEHUA TECH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IDEXX alv p27 antigen test kit
Nuclear hormone receptors activate HBV replication in 293T cells. (A) HNF4α, RXRα, and PPARα expression plasmids were transfected into 293T cells. After 72 h, mRNA expression levels were quantified by qPCR. Control cells were untransfected 293T. ATRA at 1 μmol/L and clofibric acid at 1 mmol/L, were used as ligands to activate the RXRα and PPARα nuclear hormone receptors, respectively. Plasmid pBR322‐HBV1.0 (0.5 μg) was transfected into cells together with or without nuclear hormone receptors (HNF4α 0.2 μg, RXRα 0.2 μg, PPARα 0.2 μg). (B) At 72 h after transfection, HBV cccDNA was quantified by absolute quantitative PCR, and then calculated as copies per cell. (C) HBV pgRNA was detected by RT‐qPCR. <t>(D)</t> <t>HBsAg</t> was detected by time‐resolved fluorescence. (E) HBeAg was detected by <t>ELISA.</t> (F) HBV DNA in the supernatant was extracted and quantified using an HBV DNA real‐time PCR Assay kit. Results are the means ± SD of three repeats, with differences assessed using Student's t test (* P < .05, ** P < .01, *** P < .001)
Alv P27 Antigen Test Kit, supplied by IDEXX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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AESKU Inc aeskulisa® elisa test kits
Nuclear hormone receptors activate HBV replication in 293T cells. (A) HNF4α, RXRα, and PPARα expression plasmids were transfected into 293T cells. After 72 h, mRNA expression levels were quantified by qPCR. Control cells were untransfected 293T. ATRA at 1 μmol/L and clofibric acid at 1 mmol/L, were used as ligands to activate the RXRα and PPARα nuclear hormone receptors, respectively. Plasmid pBR322‐HBV1.0 (0.5 μg) was transfected into cells together with or without nuclear hormone receptors (HNF4α 0.2 μg, RXRα 0.2 μg, PPARα 0.2 μg). (B) At 72 h after transfection, HBV cccDNA was quantified by absolute quantitative PCR, and then calculated as copies per cell. (C) HBV pgRNA was detected by RT‐qPCR. <t>(D)</t> <t>HBsAg</t> was detected by time‐resolved fluorescence. (E) HBeAg was detected by <t>ELISA.</t> (F) HBV DNA in the supernatant was extracted and quantified using an HBV DNA real‐time PCR Assay kit. Results are the means ± SD of three repeats, with differences assessed using Student's t test (* P < .05, ** P < .01, *** P < .001)
Aeskulisa® Elisa Test Kits, supplied by AESKU Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Diapro Inc elisa test kit
Nuclear hormone receptors activate HBV replication in 293T cells. (A) HNF4α, RXRα, and PPARα expression plasmids were transfected into 293T cells. After 72 h, mRNA expression levels were quantified by qPCR. Control cells were untransfected 293T. ATRA at 1 μmol/L and clofibric acid at 1 mmol/L, were used as ligands to activate the RXRα and PPARα nuclear hormone receptors, respectively. Plasmid pBR322‐HBV1.0 (0.5 μg) was transfected into cells together with or without nuclear hormone receptors (HNF4α 0.2 μg, RXRα 0.2 μg, PPARα 0.2 μg). (B) At 72 h after transfection, HBV cccDNA was quantified by absolute quantitative PCR, and then calculated as copies per cell. (C) HBV pgRNA was detected by RT‐qPCR. <t>(D)</t> <t>HBsAg</t> was detected by time‐resolved fluorescence. (E) HBeAg was detected by <t>ELISA.</t> (F) HBV DNA in the supernatant was extracted and quantified using an HBV DNA real‐time PCR Assay kit. Results are the means ± SD of three repeats, with differences assessed using Student's t test (* P < .05, ** P < .01, *** P < .001)
Elisa Test Kit, supplied by Diapro Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Abbexa Ltd elisa test kit for estradiol
Nuclear hormone receptors activate HBV replication in 293T cells. (A) HNF4α, RXRα, and PPARα expression plasmids were transfected into 293T cells. After 72 h, mRNA expression levels were quantified by qPCR. Control cells were untransfected 293T. ATRA at 1 μmol/L and clofibric acid at 1 mmol/L, were used as ligands to activate the RXRα and PPARα nuclear hormone receptors, respectively. Plasmid pBR322‐HBV1.0 (0.5 μg) was transfected into cells together with or without nuclear hormone receptors (HNF4α 0.2 μg, RXRα 0.2 μg, PPARα 0.2 μg). (B) At 72 h after transfection, HBV cccDNA was quantified by absolute quantitative PCR, and then calculated as copies per cell. (C) HBV pgRNA was detected by RT‐qPCR. <t>(D)</t> <t>HBsAg</t> was detected by time‐resolved fluorescence. (E) HBeAg was detected by <t>ELISA.</t> (F) HBV DNA in the supernatant was extracted and quantified using an HBV DNA real‐time PCR Assay kit. Results are the means ± SD of three repeats, with differences assessed using Student's t test (* P < .05, ** P < .01, *** P < .001)
Elisa Test Kit For Estradiol, supplied by Abbexa Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nuclear hormone receptors activate HBV replication in 293T cells. (A) HNF4α, RXRα, and PPARα expression plasmids were transfected into 293T cells. After 72 h, mRNA expression levels were quantified by qPCR. Control cells were untransfected 293T. ATRA at 1 μmol/L and clofibric acid at 1 mmol/L, were used as ligands to activate the RXRα and PPARα nuclear hormone receptors, respectively. Plasmid pBR322‐HBV1.0 (0.5 μg) was transfected into cells together with or without nuclear hormone receptors (HNF4α 0.2 μg, RXRα 0.2 μg, PPARα 0.2 μg). (B) At 72 h after transfection, HBV cccDNA was quantified by absolute quantitative PCR, and then calculated as copies per cell. (C) HBV pgRNA was detected by RT‐qPCR. (D) HBsAg was detected by time‐resolved fluorescence. (E) HBeAg was detected by ELISA. (F) HBV DNA in the supernatant was extracted and quantified using an HBV DNA real‐time PCR Assay kit. Results are the means ± SD of three repeats, with differences assessed using Student's t test (* P < .05, ** P < .01, *** P < .001)

Journal: Journal of Cellular and Molecular Medicine

Article Title: Defined host factors support HBV infection in non‐hepatic 293T cells

doi: 10.1111/jcmm.14944

Figure Lengend Snippet: Nuclear hormone receptors activate HBV replication in 293T cells. (A) HNF4α, RXRα, and PPARα expression plasmids were transfected into 293T cells. After 72 h, mRNA expression levels were quantified by qPCR. Control cells were untransfected 293T. ATRA at 1 μmol/L and clofibric acid at 1 mmol/L, were used as ligands to activate the RXRα and PPARα nuclear hormone receptors, respectively. Plasmid pBR322‐HBV1.0 (0.5 μg) was transfected into cells together with or without nuclear hormone receptors (HNF4α 0.2 μg, RXRα 0.2 μg, PPARα 0.2 μg). (B) At 72 h after transfection, HBV cccDNA was quantified by absolute quantitative PCR, and then calculated as copies per cell. (C) HBV pgRNA was detected by RT‐qPCR. (D) HBsAg was detected by time‐resolved fluorescence. (E) HBeAg was detected by ELISA. (F) HBV DNA in the supernatant was extracted and quantified using an HBV DNA real‐time PCR Assay kit. Results are the means ± SD of three repeats, with differences assessed using Student's t test (* P < .05, ** P < .01, *** P < .001)

Article Snippet: The supernatants were harvested and used to determine the concentrations of HBsAg and HBeAg using ELISA test kits (KeHua) following the manufacturer's instruction.

Techniques: Expressing, Transfection, Control, Plasmid Preparation, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Fluorescence, Enzyme-linked Immunosorbent Assay